IP Protocols and hints

[Bean Long]

Nick Theodorakis wrote:
> On May 23, 2:44 am, Bean Long <ben.l... from yourfinger.anu.edu.au> wrote:
> 
> 
>> Many thanks AK. I s'pose 'enrich' would be a better word? The hope is to
>> do a bit of proteomic work and subunit relative abundance analysis on
>> it. Enough to run relative pure stuff on a gel would be handy. I had a
>> little play with the Protein G-sepharsoe I found but ended up with
>> bucket loads of what I assume is Protein G on my gels. I also think it's
>> a little too old for successful IP, so I'm getting a new batch!
> 
> More likely it was IgG you saw on your gel. The heavy chains are
> between 50-60 kDa and the light chains are 20-something-ish kDa.

My first thought too Nick, but the same bands showed in my Protein-G 
only control... no IgG added!! Any recommendations for good sources of 
Protein-A or Protein-G beads for IP??

Cheers,

Bean