Help regarding FLAG IP and detergent in elution

[WS]

Maybe you can change the lysis method to something mechanical like
potter or high speed shearing (rotor-stator device): When your protein
of interest is not membrane bound or transmembrane, detergent should
not be a prerequisite to solubilize your protein, as it is already
floating in the cytoplasm, so the detergent just would disrupt the
membrane and set it free.

Wo