Methods Digest, Vol 40, Issue 19, Setting up a new mole biol laboratory

[Virash Gupta]

Add a good quality laminar flow bench, simple PCR
mchines, dedicated microcentrifuge ets as well

On 9/25/08, methods-request from oat.bio.indiana.edu <
methods-request from oat.bio.indiana.edu> wrote:
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> Today's Topics:
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>   1. RE: Setting up a new mole biol laboratory (Rory O'Brien)
>   2. Transfection myoblast (laure d'astarac)
>   3. delipidized BCS (FBS)  (Zamawang Faisel Almemar-Ray)
>
>
> ----------------------------------------------------------------------
>
> Message: 1
> Date: Wed, 24 Sep 2008 11:26:58 +1200
> From: "Rory O'Brien" <rory.obrien from stonebow.otago.ac.nz>
> Subject: RE: Setting up a new mole biol laboratory
> To: <methods from magpie.bio.indiana.edu>
> Message-ID: <E1KiHHb-0007G8-L2 from galadriel.otago.ac.nz>
> Content-Type: text/plain;       charset="US-ASCII"
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> A set of Current Protocols and/or the new(er) edition of Maniatis.
>
> and an ultrapure water supply, Milli-Q or similar.
>
> - R.
>
>
>
> > Dear Friends,
> >
> > We are planning to set up a molecular biology laboratory from
> > scratch. I am required to make a list of equipments required
> > for regular use in the laboratory. We have already
> > shortlisted major equipment like
> >
> > chemidoc system, RT PCR, 2D gel electrophoresis system,
> > laminar air flow,  etc
> >
> > with smaller equipments like submarine gel electrophoresis
> > units, PAGE units, western and southern blot apparatus, speed
> > vac, hand held uv torch, uv transilluminator, tabletop
> > centrifuge, autoclave,
> >
> > and other everyday use apparetus like waterbath, pH meter,
> > magnetic stirrers, vortex, incubator, oven, digital balance
> > and the freezers of -20 and -70 degrees Celsius etc
> >
> > I need some inputs on whether there is anything crucial from
> > the point of view of a mole biol lab that I may have missed
> > out... Any and all suggestions are welcome!
> >
> > Regards,
> > --Yg
> > _______________________________________________
> > Methods mailing list
> > Methods from net.bio.net
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>
> ------------------------------
>
> Message: 2
> Date: Wed, 24 Sep 2008 15:55:04 +0200
> From: "laure d'astarac" <lauredastarac from gmail.com>
> Subject: Transfection myoblast
> To: methods from magpie.bio.indiana.edu
> Message-ID:
>        <ba709e750809240655h4ce8e16cs62aa47498492d456 from mail.gmail.com>
> Content-Type: text/plain; charset=ISO-8859-1
>
> Hi,
>
> I have to start transfection of AON and siRNA in my lab, where nobody has
> experience...
>
> So several questions about myoblast transfection with small RNAs (or
> AONs...)
>
> 1/ what is the best transfection method to use: Electroporation Vs
> Lipfectamin Vs other?
>
> 2/ If someone has general rules about minimum and maximum amount of AON -Si
> RNA to use in transfection, in order to be efficient but not toxic... I
> suppose i have to do several quantities, but values are arround ng,
> microg???
>
> 3/ When harrest cells after transfection: 24h? 48? test both?
>
> I am sorry about these basic questions.... and thank you for your help....
>
> --
> Claire Navarro
> Stem cell laboratory
> University of Milan - Ospedale Maggiore Policlino di Milano -
> Padiglione Ponti, II piano
> Via F. Sforza, 35
> 20122 Milan
> Italy
> Tel: 00 39 02 55 03 38 52
> 00 39 02 55 03 38 74
>
>
> ------------------------------
>
> Message: 3
> Date: Tue, 23 Sep 2008 17:50:56 -0600
> From: "Zamawang Faisel Almemar-Ray" <zalmemar from uccs.edu>
> Subject: delipidized BCS (FBS)
> To: methods from magpie.bio.indiana.edu
> Message-ID: <web-80101311 from uccs.edu>
> Content-Type: text/plain;charset=iso-8859-1;format="flowed"
>
> Could someone please tell me how to make delipidized BCS (FBS) using
> charcoal stripping. I desparately need the procedure for this, as my
> research is on hold because my order for delipidized BCS (FBS) is on
> back-order.
>
> Thanks,
> Z
>
>
>
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> End of Methods Digest, Vol 40, Issue 19
> ***************************************
>



-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210