RNA extraction

Nick Theodorakis via methods%40net.bio.net (by nick.theodorakis from gmail.com)
Mon Apr 20 10:46:23 EST 2009


On Apr 20, 2:03 am, y... from deakin.edu.au wrote:
> Hello,
>
> Just wondering if there is any way to extract RNA, prefer just the  
> rRNA, of e. coli without losing the proteins?

Are you asking how to isolate ribosomes from E. coli? There are
probably at least dozens of procedures one can find with a MEDLINE
search, but most will probably have a centrifugation step in an
ultracentrifuge; further clean up will depend in your specific needs.
Can you give us a little more detail on what you are trying to do?

Nick

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Nick Theodorakis
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