Methods Digest, Vol 47, Issue 16

[Tom Knight]

novalidaddress from nurfuerspam.de writes:

> As the DNA is not in the chloroform phase, it is in the aqueous
> phase which contains the phenol (i.e. the phenolic phase),
> too. Maybe I haven't expressed that clearly enough (I wrote "phenol
> phase" which of course is not correct, there is no separate phenol
> phase, of course.

The majority of the phenol partitions along with the chloroform phase.
Small amounts of phenol partition into the aqueous phase, along with
the DNA.  A subsequent extraction of the aqueous phase with chloroform
is used to remove most of the remaining phenol from the aqueous phase,
followed typically with ethanol preciptiation.