DNA software question

[chovek69]

On Jan 13, 5:04 am, Nick Theodorakis <nick.theodora... from gmail.com>
wrote:
> Han wrote:
> > Colleague at Cornell Medical College has a very old Mac with an old version
> > of DNAStar, which he likes very much.  His use is not very intensive, but
> > primer design, sequence alignment etc are used relatively often.
>
> > Since the Mac is about to die, he would like software for a PC (XP Pro)
> > that could best perform those functions.  DNAStar (new version) costs
> > $1,000/year in licensing costs, which colleague doesn't regard as a wise
> > investment.  What are his best options for free or cheap software with
> > similar capabilities as what he wants of DNAStar (primer design, sequence
> > alignment, etc)?
>
> For primer design, I and nearly everyone I know use Pimer3. There is a
> web version:
>
> <http://frodo.wi.mit.edu/primer3/input.htm>
>
> which is also now built into the NCBI primer blast page:
>
> <http://www.ncbi.nlm.nih.gov/tools/primer-blast/index.cgi?
> LINK_LOC=BlastHome>
>
> Downloadable source is also available:
>
> <http://primer3.sourceforge.net/>
>
> --
> Nick Theodorakis
> nick_theodora... from hotmail.com
> contact form:http://theodorakis.net/contact.html

and for seq alignment Bioedit and MEGA 4...