Low concentration miniprep

[David-Paul Minde]

Hi Yasir

> is your gene very large?what kit are you using? if the gene (and so the
> plasmid) is large, maybe you should try to increase the quantity of the
> bacteria when doing miniprep...

e.g. Terrific Broth would help in this case (and if still necessary) bigger
volumes of culture.
Of course you shold not exceed the specs of your kit (or you will have
decrease of DNA quality)
best,
David

>
>
> 2009/1/14 muhammad yasir <yasirphr from yahoo.com>
>
> > I have clone the gene in pUC19 and get conformation with restriction
> > digestion. But unfortunately, I get very low quantity of plasmid after
> > miniprep. While the control (circular pUC19) concentration is quite
> enough
> > with miniprep kit that I used. I shall be very glad to know about the
> > possible reason of low concentration and how can I improve the yield of
> > clone plasmid from miniprep.
> >
> > Yasir
> >
> >
> >
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>
>
> --
> Fulvio Celsi
> BsC,PhD
> Sector of Neurobiology,
> International School for Advanced Studies, Scuola Internazionale di Studi
> Superiori Avanzati (SISSA),
> Trieste
> Italy
>
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-- 
David Minde MSc (TUM)

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