Standard Curve qPCR

[Yvonne Couch]

Hi all,

I have come against some contention as regards making a standard curve for
qPCR.  My current method is to take a known amount of cDNA (e.g. 40ng/ul)
from each sample (so 2ul from 10 samples = total of 800ng) then dilute so I
have 100ng total cDNA in my top standard, 50ng in the next, 25ng in the
next, etc.  However, a colleague PCRs up the exact region she needs (e.g.
IL6) and then dilutes that as a standard for each set of primers.  I was
told the way I do things would be frowned upon if I tried to publish.  Any
thoughts?

Cheers!