storing of enzyme
Nick Theodorakis
via methods%40net.bio.net
(by nick.theodorakis from gmail.com)
Tue Jul 28 16:55:30 EST 2009
On Jul 28, 3:51 pm, "Dr. Hiranya S. Roychowdhury" <hroyc... from nmsu.edu>
wrote:
> Use a buffer (10mM Tris-EDTA, pH 6.5 to 7.2, or 50mM phosphate buffer
> should work) and add glycerol to 35% (as high as 50%). Store in a -20C
> freezer (no auto defrost).
>
> Cellulase can also be stores freeze dried after the appropriate dialysis
> against distilled water containing 1mM DTT.
>
Is dialysis tubing still made from cellulose?
Nick
--
Nick Theodorakis
nick_theodorakis from hotmail.com
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