Promoter amplification

[Christian Praetorius]

Peter Ellis <pjie2 from cam.ac.uk> wrote:

>Do you have to PCR it, 

No, I don't have to. Its simply easier, when it works.

>or could you cut a restriction fragment out of a 
>BAC prep and clone that instead?

Good sugestion. I am actually digging into it, and it seems possible.
But I have to change the MCS of my plasmid but inserting a oligo with
the right sites. 

Christian

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