Total Protein

M Aldeeb via methods%40net.bio.net (by moh_aldeeb from yahoo.com)
Tue Jun 9 05:12:24 EST 2009


Dear All,

I have a spectrophotometer (with cuvette). I want to measure total protein using BioRad Dye (BradFord method). How much protein (insect homogenate)and how much dye?

Is it 100 uL Protein + 1900 uL (1X) Dye? @ 595 nm

Please advise. Many thanks.

Mohammad Ali Al-Deeb



      



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