agarose electrophoresis tank @ $10

[Dr. Hiranya S. Roychowdhury]

We did that some 15 years back!


> You might enjoy this article in the Science Creative Quarterly, which
> explains how to use available-at-supermarket ingredients to perform DNA
> extractions, gel electrophoresis and staining.
> http://is.gd/1hPJs
> The short answer is: you can use agar, though the band will be a little
> blurry, and you can use a blue food colouring to stain the DNA, the
> specific
> name is in the article. Your agar might need to be good and pure, but if
> it's just to demonstrate the principal of electrophoresis it's fine.
>
> 2009/6/26 Dr Engelbert Buxbaum <engelbert_buxbaum from hotmail.com>
>
>> Am 26.06.2009, 13:21 Uhr, schrieb aria johnson <ariajohnson from gmail.com>:
>>
>>  Hi Check this website out for simple tank
>>> http://www.science-projects.com/GE/HorizChamber.htm
>>> not sure whether agar will give you the resolution you need for the
>>> exercise.
>>> Will you use EtBr and UV for visualization? Im not sure what age is 6th
>>> form
>>> but you may want to monitor the EtBr step or find an alternate way
>>> to visualize your pcr product.
>>> take care
>>> aj
>>>
>>> On Fri, Jun 26, 2009 at 6:23 AM, mccraight <
>>> mccraight from biddulph.staffs.sch.uk
>>>
>>>> wrote:
>>>>
>>>
>>>  Hi, I am a Lab technician in a school,and we need an electophoresis
>>> tank
>>>> to
>>>> enable our 6th for students to follow a new  forensic science course.
>>>> Money
>>>> in school is very limited,so I am hoping to make one. Would it be
>>>> possible
>>>> for you to send me details on how to construct a simple tank,and
>>>> whether
>>>> ordinary agar could be used instead of agarose as this would also cut
>>>> costs.
>>>>
>>>
>> Agarose for electrophoresis has been purified to contain few if any
>> charged
>> groups, which would cause a phenomenon called electroendosmosis. This
>> can
>> seriously degrade resolution. Exception: Counter-stream
>> immunoelectrophoresis and certain modes of capillary electrophoresis,
>> where
>> electroendosmosis is actually required for separation.
>>
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-- 
Hiranya S. Roychowdhury, Ph.D.
Asst. Professor,
Health & Public Services
Dona Ana Community College
New Mexico State University
Las Cruces, NM 88003