2D gel or MS? for comparison proteomic

[Dr Engelbert Buxbaum]

Am 25.02.2009, 12:29 Uhr, schrieb Zhong Silin <stxsz1 from nottingham.ac.uk>:


> I want to compare total protein level from two types of plant cells.  
> Since my lab is made up of geneticists, I dont think we can do this  
> experiment on our own. I would prefer to find a company who provide such  
> services or collaborate with some other proteomic groups.
>
> Before contacting them, could some one give me some idea about this type  
> of experiment? I only know people have used 2D gel and MS/MS to compare  
> protein levels in arabidopsis roots.

It is not either/or, but one after the other. First you separate the  
proteins, then you analyse by MS/MS. The classic method for separation is  
2D-electrophoresis, but multi-dimensional chromatography (look for  
"MuDPIT") has also been described.