Methods Digest, Vol 46, Issue 6

[Virash Gupta]

inactivate CIAP in the presence of 2.5mM EDTA at 65C then clean with Qiaex.

On 3/7/09, methods-request from oat.bio.indiana.edu <
methods-request from oat.bio.indiana.edu> wrote:
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>   1. Re: Genomic library construction troubleshooting (DK)
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> Message: 1
> Date: Fri, 06 Mar 2009 22:23:37 GMT
> From: dk from no.email.thankstospam.net (DK)
> Subject: Re: Genomic library construction troubleshooting
> To: methods from net.bio.net
> Message-ID: <gos7p3$5n9$1 from news.albasani.net>
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> In article <mailman.423.1236284202.13724.methods from net.bio.net>,
> "=?ISO-8859-1?Q?Rosario_D=EDaz?=-=?ISO-8859-1?Q?Gonz=E1lez?="
>        <rdiazg from ipb.csic.es> wrote:
> >Thanks all for the tips. Unfortunately, I can't not ride off of CIAP since
> my
> > IP is really fond of with that enzyme, don't tell him about trying SAP.
>
> Does he want to just stick to his principles or get the job done?
> Leave out CIAP. In 90% cases it creates more problems than it
> solves.
>
> DK
>
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> End of Methods Digest, Vol 46, Issue 6
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-- 
Dr V K Gupta
Sr Microbiologist (Molecular Biology)
Insect Molecular Biology Lab
Department of Entomology
Punjab Agricultural University
Ludhiana (Pb)-141004- India
M: 09815963210