method for membrane receptor extraction

ketut gunarta via methods%40net.bio.net (by duadjuz from yahoo.com)
Wed Mar 3 06:38:58 EST 2010


Hello all,
I have to isolate G-PCR (G-protein coupled receptor) from ovarian cancer cell line. I am looking for a protocol (buffer,etc) that not use extraction/isolation kit (I could mix the buffer by my self) and not change its native structure. There is a problem that my ultracentrifuge only can spin up to 15,000 g. I hope anybody could help me. Thank you very much.


      


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