Cotransformation

[Kyle Legate]

waikhay wrote:
> Hi all! :)
> 
> I would like to transform 2 plasmids i.e. an expression vector (carrying 
> the
> gene of interest) and a reporter vector (into which the promoter of 
> interest
> has been cloned) into BL21(DE3) cells. Would you recommend/suggest
> transforming both plasmids into the cells at once (cotransform) or
> transforming one plasmid after another (i.e. transform the reporter vector
> into the cells first, select for transformants, then only transform the
> expression vector and select for cotransformants)?
> 
You can do them both at the same time as long as they have different 
antibiotic resistances and compatible origin

s.