Trypsin/EDTA

[Lara]

Hello there folks,

I use for some endothelial cells trpsin/edta and according to the
protocol I have to pipette in 5 ml trypsin/edta and then immediately
remove 4.5 ml and leave the rest for a couple of minutes. Does anyone
know why? Isn't this simply a waste of trypsin?

Furthermore, is there any place where I can find detailed info about
trypsin in particular? Thanks for any info.

Cheers,
Lara