Trypsin/EDTA

[Kyle Legate]

Lara wrote:
> Dear DK and Han,
> 
> thanks so much for your helpful feedback. Indeed, now I understand it
> is not a waste of either Trypsin or EDTA. With responses like this
> newsgroups get another dimension in science. Thank a lot again!
> 
It most certainly is a waste of trypsin/EDTA. If what DK says is true, 
and the extra solution is to dilute serum-containing medium, you can 
achieve the same goal by washing the plate once with PBS and then adding 
a smaller volume of trypsin. Here is what I do for 10 cm plates or 75 
cm2 flasks: aspirate medium, wash once with 10 ml PBS, aspirate, add 1 
ml trypsin/EDTA and place in the incubator for 5 min, or until cells 
detach. I have never had a problem.