Trypsin/EDTA

[Lara]

> Washing with PBS is preferable because it more effectively removes the
> inhibitors (as you don't leave any behind in the flask when you aspirate
> all the PBS), and washing with PBS first removes dead cells and debris
> much more effectively.

I am not denying any of what has been written so far but you have to
apologize if I might ask you what constitutes chemically the fact that
it is more "effective". The expression more effective seems to me a
bit too broad or if you want too vague. After trypsinization you
neutralize with HBBS and remove so therefore you leave no traces
behind. The question here is not what is more economical but what does
better to the cells and since we all are interested in what is best
for our cells we stand on the same site, right?

Lara