enzyme activity calculation-clarification

[SIVAKUMAR]

Hi
Does any one could clarify the correctness of the enzyme activity 
unit calculation?


			   ???526/min x Reaction vol.				
Enzyem activity (U / ml)= ____________________________	x dilution 
factor		
			   0.065x Enzyme vol. 				
								
For e.g., 		0.03x0.2					
			_________x5#	= 9.2 U /ml or 153.36 nanokatals/ml
			0.065x0.05				
								
where, Change in abs at 526 per min was 0.03
Assay volume 0.2 ml
Enzyme volume 0.05 ml
Extn coeff: 					
 =  65,000 /M/cm					
 =  65 /Mm/cm					
 =  0.065 /uM/cm*					
 * used 0.065, since EU is defined u mole of substrate oxidized.	
5#					
since the assay volume was 0.2ml, I multiplied the whole by 5 to 
get per ml value
Is it correct?