enzyme activity calculation-clarification

[SIVAKUMAR]

  Hi
Does any one could clarify the correctness of the enzyme activity 
unit calculation?


                      526/min x Reaction vol.
Enzyem activity(U/ml)=______________________x dilution factor
                        0.065x Enzyme vol.

For e.g.,                 0.03x0.2
                        _________x5#     = 9.2 U /ml or 153.36 
nanokatals/ml
                        0.065x0.05

where, Change in abs at 526 per min was 0.03
Assay volume 0.2 ml
Enzyme volume 0.05 ml
Extn coeff:
=  65,000 /M/cm
=  65 /Mm/cm
=  0.065 /uM/cm*
* used 0.065, since EU is defined u mole of substrate oxidized.
5#
since the assay volume was 0.2ml, I multiplied the whole by 5 to 
get per ml value
Is it correct?
Siva