DNA crosslinking on nylon memberane

[Kyle Legate]

Peter Ellis wrote:
> TC wrote:
>> Hello,
>>
>> I am trying to crosslink DNA to nylon membrane for southern blots.  
>> Besides
>> traditional UV crosslinking (may induce DNA damage) and baking (causes 
>> DNA
>> to denature), are there any other way to crosslink DNA onto nylon
>> memberanes?  For my purpose, I hope minimize DNA damage and keep that 
>> DNA in
>> native condition for the Southern blots.
> 
> Why do you want your DNA in native condition?  Unless you denature it, 
> it won't be accessible for your probe to bid to and you will have 
> reduced signal.
> 
Not to mention that the type of DNA damage induced by UV does not 
inhibit hybridization efficiency one bit.

Whoops, I mentioned it.

UV crosslink the nylon filter, bake the nitrocellulose filter. That's 
how it's done.