statistical question on sequencing project

[Klaus May]

On Jul 27, 2:46 pm, WS <novalidaddr... from nurfuerspam.de> wrote:
> Dear Experts,
>
> stupid scientist wants to quantify some gene expression in his samples
> with pyrosequencing. The technology he plans to use offers him the
> possibility to identify a certain number (N) of transcripts per sample
> (at least 10.000).
>
> He has some ideas on how many different transcripts there might be
> (about 100) in one sample. Now, he is looking for a method that tells
> him how to calculate the number of sequences he has to obtain per
> sample in order to identify transcripts of which the expression is
> different from sample to sample.
>
> Example: If there is a transcript present at 1% in one sample, how
> many transcripts does he need to identify and count in order to detect
> a change in its relative expression of e.g. 100% (i.e. presence of 2%
> or 0.5% in another sample with a certain probability (say p<0.05)?
>
> Looking forward to your suggestions (textbook, paper, weblink, how
> this statistical problem is named etc.)
>

Hi!

Have a look at this science paper, especially the supplementary
material
http://www.sciencemag.org/cgi/content/abstract/1160342

There are some cosiderations about sequencing depth.

Cheers