Western Stripping Buffer?

[Kamalian, Laleh]

I have used the following composition for stripping buffer.  It has worked for me.
To make 500 ml of Stripping Buffer:
62.5 mM Tris (3.8 grams of MW 121.1)
100 mM 2-mecaptoethanol ( 3.5 ml)
2% SDS (10 grams)
PH to 6.7
Volume to 500 ml with distilled water.
I usually incubate my membrane for 1/2 hour at 50'C on a shaker plate.
Hope it helps.
Laleh


 
 

________________________________

From: methods-bounces from oat.bio.indiana.edu on behalf of John Leonard
Sent: Thu 19/06/2008 22:50
To: methods from magpie.bio.indiana.edu
Subject: Western Stripping Buffer?



Does anyone have a favorite buffer they have used to strip western blots for
re-probing?  I've found a number of recipes online, but I don't know which
to choose.  If you know of one that you particularly enjoy, please share!


Thanks,

John
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