Two media are routinely used: Green Top Agar and Peptone Yeast Extract or
Meat Extract agar with Sodium Acetate.
Green Top Agar per liter:
Yeast Extract 2 gm
Tryptic Pepone 1 gm
Sodium Acetate 1 gm
Soil Extract 200 ml
Agar 15 gm pH 7.4
Soil Extract 75 gm African Violet Soil in 200 ml
D.I. water.
0.5 gm Sodium Carbonate 0.5 gm
Autoclave 2 Hrs -
Filter
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Peptone Yeast Extract or Meat Extract Agar
Tryptic Peptone 1 gm
Yeast Extract 2 gm
or
Beef Extract 2 gm
Sodium Acetate 1 gm
Agar 15 gm
pH = 7.5
==========================================================
Additional recommended supplements:
glutamic acid thiamine biotin ammonium phosphate
l-cysteine
The prepared media or sterile extracts are available from
Randolph Biomedical - 401-826-1407.
================================================================
Robert Randolph I
Randolph Biomedical I Second Place
1-401-826-1407 I makes you the
First loser. randbio at aol.com I
rand777.ids.net I
