Dear Netters
Can someone give me any information on the use of PCR to detect
Fibrobacter succinogenes and other anaerobic cellulolytic bacteria from the
bovine rumen.
The protocol which I use requires 65 cycles to form a product(500bp)
that can be detected by electrophoresis and staining with EtBr. Also the
primer concentration is a high 200 umol/L compared to the usual 0.05 to
1.6umol/l.
The forward primer is 24 bases long and is a rDNA specific sequence
described by Aman et al 1990 J.Bac.172:762-770.
The reverse primer is an universal E.coli rDNA sequence.
We are hope to use this protocol as a lab exercise in our 4th year
Microbial Ecology course.
Thanks in advance
Rohan van Twest
Dept of Microbiology
University of Guelph
Guelph Ontario
Canada
