Hi Bionetters,
trying to work out a propper protein purification I was just wondering
which protease inhibitors would be appropriate. Using a trypsine inhi-
bitor for example does not seem to be reasonable to me, since bacteria
probably don't have those acidic systems. I'm working with cyanobacteria
and prochlorophytes and made a literature research. However, no papers
had apparently been published in the last 10 years, dealing explicitely
with this matter. People usually use a cocktail” of different inhibitors
(if ever), but I would like to know which ones are really needed.
Any help is greatly appreciated,
Wolfgang.
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| Wolfgang Doerner, Fachbereich Biologie/Botanik |
| Department of Biology/Botany |
| Karl-von-Frisch Strasse |
| Philipps-Universitaet Marburg |
| D - 35032 Marburg, Germany |
| E-mail: doerner at mailer.uni-marburg.de |
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