taylor (patel_p at bms.com) wrote:
: Hi
: I had a couple of questions about the bacteriophage lambda
: repressor binding site:
: 1) The DNA sequence for which the repressor has the highest affinity
: 2) Is the affinity increased by the presence of 2 repressor binding
: sites in tandem, and if so, the ideal distance in bp between the two
: tandem sites.
: Any suggestions or refrence leads will be appreciated.
: Thanks in advance
: Prem
: e-mail: Patel_P at BMS.com
A good place to start would be to look at a book by Mark Ptashne, called
'A genetic switch' published by Blackwell Scientific Press (I don't have
it in front of me but I think I'm correct). A new edition came out last
year. Mark Ptashne has done a lot of work on the lambda repressor so
looking at some of his papers would also be a good idea (especially the ones
published in Cell.
Vince Mulholland.
