I use tetrazolium red to indicate metabolic activity of micro-organisms
(Pseudomonads) on certain carbon sources. Some references point to the
usefulness of this method, e.g. J.Lederberg showed that:
- bacteria grown on non- acidifying carbon sources in the agar plate turn
tetrazolium red to a red color;
- bacteria grown on acidifying carbon sources (fermentation: glucose,
lactose) do not convert terazolium
My problem is, that I seem unable to find out, or at least get some
literature, on the bacterial enzymes and reactions that convert
tetrazolium. Lederberg wrote about a "dehydrogenase".
Can anyone give some hints where to look in literature ??
Just wondering what I'm looking at......
thanks in advance,
Institute for molecular plant sciences
Leiden, the Netherlands
SBY3MS at RULSFB.LEIDENUNIV.NL