In article <3miujb$rht at highway.LeidenUniv.nl>
Email (YourName) writes:
>> I use tetrazolium red to indicate metabolic activity of micro-organisms
> (Pseudomonads) on certain carbon sources. Some references point to the
> usefulness of this method, e.g. J.Lederberg showed that:
>> - bacteria grown on non- acidifying carbon sources in the agar plate turn
> tetrazolium red to a red color;
> - bacteria grown on acidifying carbon sources (fermentation: glucose,
> lactose) do not convert terazolium
>>> My problem is, that I seem unable to find out, or at least get some
> literature, on the bacterial enzymes and reactions that convert
> tetrazolium. Lederberg wrote about a "dehydrogenase".
>> Can anyone give some hints where to look in literature ??
>>> Just wondering what I'm looking at......
>> thanks in advance,
>> Marco Simons
> Institute for molecular plant sciences
> Clusius laboratory
> Leiden University
> Wassenaarseweg 64
> Leiden, the Netherlands
>>SBY3MS at RULSFB.LEIDENUNIV.NL
Tetrazolium is acting as a pH indicator.
Michael Benedik benedik at uh.edu
University of Houston
Houston, TX 77204-5934