In article <3mgir5$hd at surz03.HRZ.Uni-Marburg.DE> doerner at papin.HRZ.Uni-Marburg.DE (Doerner Wolfgang) writes:
>trying to work out a propper protein purification I was just wondering
>which protease inhibitors would be appropriate. Using a trypsine inhi-
>bitor for example does not seem to be reasonable to me, since bacteria
>probably don't have those acidic systems. I'm working with cyanobacteria
>and prochlorophytes and made a literature research. However, no papers
>had apparently been published in the last 10 years, dealing explicitely
>with this matter. People usually use a cocktail” of different inhibitors
>(if ever), but I would like to know which ones are really needed.
>Any help is greatly appreciated,
>Wolfgang.
How about bacitracin-based? You might look articles by O.V. Mosolova et al.
Biokhimiya (Biochemistry USSR), 1987, 52:414 and 52:1709 or any other
from the lab of V.M.Stepanov.
Michael Galperin galperin at biotek.mcb.uconn.edu
Dept. Molec. Cell Biol., U-125 (203) 486-4330 (lab)
The University of Connecticut (203) 486-4331 (fax)
Storrs, CT 06269-3125
-
