I have been working for five years on the proteolytic system of
Lactobacillus delbrueckii subsp. bulgaricus, including a lot of
biochemistry, genetic and molecular biology. Actually, I am working as a
research and teaching associate in the university of Lyon and I am
looking for a postdoctoral position beginning from september or october
1995. I am interested to work in lots of field of research, including all
these technical skills.
You can reply to my e-mail address : gilbert at biomserv.univ-lyon1.fr
Best regards,
Dr. Christophe Gilbert
CURRICULUM VITAE
Christophe GILBERT
PERSONAL DATA
Date of birth July, 8th, 1966 (age 27)
Place of birth Cherbourg, Manche, France
Nationality french
Marital status single
Home address 81, rue Magenta
69100 Villeurbanne
France
University address Laboratoire de Microbiologie et Génétique
Moléculaire
Bâtiment 405, 43 Bd du 11 novembre 1918
69622 Villeurbanne cedex - France
Home number (33) 78 85 18 61
University number (33) 72 43 13 66 FAX : (33) 72 43 11 81
e-mail : gilbert at biomserv.univ-lyon1.fr
EDUCATION
1984 : Baccalaureat C (Maths and Physics) with AB distinction - French
equivalent to the Higher Leaving Certificate
1984 - 1989 : Institut National des Sciences Appliquées in Lyon, a
leading general engineering school in France (five years long)
1989 : Engineer's Degree in Biochemistry
1990 : Diplôme d'Etudes Approfondies in Cellular Differentiation, Genetic
and Immunology with B distinction - equivalent to a Postgraduate Degree
Claude Bernard University - Lyon I -France
1993 : Ph. D Degree in Genetic and Microbiology
Claude Bernard University - Lyon I -France
APPOINTMENTS
september 1990 - august 1993 : Research Fellowship - Recipient of
fellowship from the Centre National de la Recherche Scientifique (CNRS),
France
september 1993 - august 1995 : Research and Teaching Associate - Claude
Bernard University - Lyon I, France
RESEARCH EXPERIENCE
Laboratoire de Microbiologie et Génétique Moléculaire
Professor R. PORTALIER
(Centre de Génétique Moléculaire et Cellulaire - CNRS, UMR 106)
Université Claude Bernard - Lyon I
1989 - 1995 : Genetic, physiological and biochemical analysis of the
proteolytic system of Lactobacillus delbrueckii subsp. bulgaricus
Technical skills : Biochemistry (proteins purification : chromatography,
HPLC, IEF, SDS-PAGE... ; enzymatic studies...) ; Genetic (mutants
isolation, conjugaison, transduction, transformation, electroporation,
heterologous gene expression...) ; Molecular Biology (gene bank, southern,
northern, DNA sequencing, and computer analysis...) ; Microbiology
(studies and work with Escherichia coli, different strains of Lactococcus
and Lactobacillus)
FOREIGN LANGUAGE ABILITY
English : working knowledge
PUBLICATIONS AND COMMUNICATIONS
PUBLICATIONS
- P. Laloi, D. Atlan, B. Blanc, C. Gilbert and R. Portalier (1991) Cell
wall associated proteinase of Lactobacillus delbrueckii subsp. bulgaricus
CNRZ397 : differential extraction, purification and properties of the
enzyme. Applied Microbiology and Biotechnology, 36 : 196-204.
- B. Blanc, P. Laloi, D. Atlan, C. Gilbert and R. Portalier (1993) Two
cell wall-associated aminopeptidases from Lactobacillus helveticus and the
purification and characterization of APII from strain ITGL1. Journal of
General Microbiology, 139 : 1441-1448.
- D. Atlan, C. Gilbert, B. Blanc, J. Frot-Coutaz and R. Portalier.
Proteolytic system of lactobacilli (1993). Proteolytic system of
lactobacilli in "The Lactic Acid Bacteria". Ed. E. L. Foo, H. G. Griffin,
R. Mollby and G. G. Heden.
- C. Gilbert, D. Atlan, B. Blanc and R. Portalier (1994) Proline
iminopeptidase from Lb. delbrueckii subsp. bulgaricus CNRZ397 :
purification and characterization. Microbiology, 140 : 537-542.
- D. Atlan, C. Gilbert, B. Blanc and R. Portalier (1994) Cloning,
sequencing and characterization of the pepIP gene encoding a
proline-iminopeptidase from Lactobacillus delbrueckii subsp. bulgaricus
CNRZ397. Microbiology, 140 : 527-535.
- C. Gilbert, B. Blanc, D. Atlan and R. Portalier (1995) Isolation and
characterisation of proteinase-deficient mutant from Lactobacillus
delbrueckii subsp. bulgaricus CNRZ397 unable to degrade caseins (in
preparation)
- C. Gilbert, B. Blanc, R. Portalier, D. Atlan, L. Lapierre, J. E. Germond
and B. Mollet (1995) The cell surface proteinase of Lb. bulgaricus :
sequencing of the structural prtB and analysis of characteristics
different from lactococcal proteinases (PrtP) (in preparation)
COMMUNICATIONS
- P. Laloi, D. Atlan, B. Blanc, C. Gilbert, R. Portalier, D. Hartley. Le
système protéolytique de Lactobacillus delbrueckii subsp. bulgaricus.
First scientific day of "centre international de recherche Daniel
CARASSO", Le plessis Robinson - France, 27 april 1990.
- P. Laloi, D. Atlan, B. Blanc, C. Gilbert, R. Portalier. Purification and
characterization of a cell-wall associated proteolytic enzyme from L.
delbrueckii subsp. bulgaricus. ASM Annual Meeting. Dallas, USA. 5-9 may
1991.
- D. Atlan, C. Gilbert, B. Blanc, R. Portalier. Clonage, séquençage et
expression chez Escherichia coli du gène codant pour la proline
iminopeptidase de Lactobacillus delbrueckii subsp. bulgaricus. Third
Meeting of "Société Française de Microbiologie", Lyon - France, 21-24
april 1992.
- C. Gilbert, D. Atlan, B. Blanc, R. Portalier. Purification et
caractérisation de la proline iminopeptidase de Lactobacillus delbrueckii
subsp. bulgaricus surproduite chez E. coli K12. 5th Meeting of "Club des
Bactéries Lactiques" : CBL-92, Nancy - France, 2-4 september 1992.
- D. Atlan, C. Gilbert, B. Blanc, J. Frot-Coutaz and R. Portalier.
Proteolytic system of lactobacilli. 1st Lactic Acid Bacteria Computer
Conference. may-june 1993.
- D. Atlan, C. Gilbert, B. Blanc and R. Portalier. The proline
iminopeptidase of Lactobacillus delbrueckii subsp. bulgaricus : cell wall
location, purification, cloning and characterization of its structural
gene. Fourth symposium on lactic acid bacteria. Genetics, Metabolism and
Applications. Noordwijkerhout, The Netherlands, 5-9 september 1993.
- J. Frot-Coutaz, C. Gilbert, D. Atlan, B. Blanc and R. Portalier.
Purification and partial characterization of the prolyl-dipeptidyl
aminopeptidase of Lactobacillus delbrueckii subsp. bulgaricus. Fourth
symposium on lactic acid bacteria. Genetics, Metabolism and Applications.
Noordwijkerhout, The Netherlands, 5-9 september 1993.
- J.E. Germond, L. Lapierre, M. Delley, D. Atlan, B. Blanc, C. Gilbert, R.
Portalier. Cell wall bound proteinase in Lactobacillus delbrueckii subsp.
bulgaricus : isolation and study of the gene.The 6th Meeting of "Club des
Bactéries Lactiques", Université Lyon I - France, 27-29 April 1994.
- J. Frot-Coutaz, C. Gilbert, D. Atlan, B. Blanc, R. Portalier.
Purification et caractérisation d'une activité aminopeptidase P chez
Lactobacillus delbrueckii subsp. bulgaricus The 6th Meeting of "Club des
Bactéries Lactiques", Université Lyon I - France, 27-29 April 1994.
- C. Gilbert, B. Blanc, D. Atlan, J. Frot-Coutaz, R. Portalier.
Identification et analyse d'un mutant de Lactobacillus delbrueckii subsp.
bulgaricus deficient pour l'activité protéinasique et incapable de
dégrader les caséines. The 6th Meeting of "Club des Bactéries Lactiques",
Université Lyon I - France, 27-29 April 1994.
- B. Blanc, D. Atlan, C. Gilbert, J. Frot-Coutaz, R. Portalier. Analyse
moléculaire de la proline iminopeptidase de Lactobacillus delbrueckii
subsp. bulgaricus CNRZ397 : identification d'acides aminés impliqués dans
l'activité enzymatique. The 6th Meeting of "Club des Bactéries Lactiques",
Université Lyon I - France, 27-29 April 1994.
- L. Lapierre, C. Gilbert, B. Blanc, D. Atlan, J.E. Germond; B. Mollet, R.
Portalier. The cell-surface proteinase of Lactobacillus delbrueckii subsp.
bulgaricus : identification and characterization of structural and
regulator genes. The 6th Meeting of "Club des Bactéries Lactiques",
Université Lyon I - France, 27-29 April 1994.
