Help on Bacterial ID !!

Peter J. Harris assharrp at reading.ac.uk
Mon Dec 11 11:16:14 EST 1995

Hello Jacqui.
You are right in suspecting a broad group. The conventional wisdom says 
that Corynebacteria, (Arthrobacter), Mycobacteria, (Streptomyces) and 
Pseudomonas are the first candidates with the latter group predominant. 
You can get some way with just straight microscopy. Worth looking at the 
colonies on the plates as well in case there are some Nocardia there. It 
might be possible to try a suitable G-ve API set on any you "think" might 
be Pseudomonas, just to see what you get. Worth getting fresh isolates 
preserved as soon as you are sure that they are pure as fresh 
environmental isolates may well shed characters when continually subbed 
in the lab. 
The daftest hydrocarbon utiliser I have seen mentioned was
"an achlorophilous alga" - though how you recognise it as such I have no idea! 

Peter Harris,
Reading, UK.

On Mon, 11 Dec 1995, Jacqui wrote:

> Hello,
> I read your reply on Bacterial i.d. I'm currently trying to identify some 
> hydrocarbon utilising organisms which have been isolated from 
> environmental samples (soil and sediments). I don't really Know where to 
> start, as you'll know this metabolic capacity is common to a large and 
> heterogenous group. If you could point me in the right direction I'd be 
> really grateful. Thanks. Jacqui Shand, Phd Student, University of York.

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