glycerol vs glucose

Forday Wayne Lee fwl at titan.np.ac.sg
Mon Dec 11 02:22:32 EST 1995

In article <DJ8HBE.5ys at cix.compulink.co.uk>,
:>> Super-rich media recipes for growth of E coli typically include 
:>glycerol but
:>> not glucose.  Anyone know why?
:>> Thanks,  Pete
:>This is not an answer but is there a possibility that it is connected 
:>with water activity ?
:>Peter Harris,
:>University of Reading, U.K.

It may be something to do with the Glucose effect. Under high glucose
concentrations, E. coli switches to fermentation mode, regardless of the
oxygen concentration. I think the factor most influential is the
concentration of FDP. Perhaps glycerol leads to lower FDP concentrations and
thus pushes the cells to respire.

An interesting experiment.

Wayne Lee Forday, Biotechnology Department 
Ngee Ann Polytechnic, Singapore 
fwl at np.ac.sg

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