In article <DJ8HBE.5ys at cix.compulink.co.uk>,
:>>:>> Super-rich media recipes for growth of E coli typically include
:>glycerol but
:>> not glucose. Anyone know why?
:>>:>> Thanks, Pete
:>This is not an answer but is there a possibility that it is connected
:>with water activity ?
:>Peter Harris,
:>University of Reading, U.K.
:>
It may be something to do with the Glucose effect. Under high glucose
concentrations, E. coli switches to fermentation mode, regardless of the
oxygen concentration. I think the factor most influential is the
concentration of FDP. Perhaps glycerol leads to lower FDP concentrations and
thus pushes the cells to respire.
An interesting experiment.
--
Wayne Lee Forday, Biotechnology Department
Ngee Ann Polytechnic, Singapore
fwl at np.ac.sg
Fax:467-9109
