Mark Mark
Thu Dec 14 17:50:41 EST 1995

In article <4apcc2$7bb at post.tau.ac.il> il <f139994 at ccsg.tau.ac.il> writes:
>I have a simple question: is it possible to store vegetative culture of 
>B.subtilis in +4C (on the plate / liqued medium), how long?
>Thank you in advance.
B.subtilis do not like 4C storage - they die. You can store them on plates
wrapped in parafilm or like for a number of weeks after they have sporulated.
We store spores on slopes at 4C for long periods(years). Simply make a slope,
inubate for a few days. Check under the microscope that youhave a good number
of spores, then seal it tight and store at 4C.
All the best
Mark Smith                      | The saying is sure and worthy of
Dept. of Biochemistry           | full acceptance, that Christ Jesus
University of Sydney            | came into the world to save sinners.
Sydney, NSW, 2006               | And I am the foremost of sinners.
Austraila                       | 
m.smith at biochem.usyd.edu.au     | 1 Timothy 1:15

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