In article <4apcc2$7bb at post.tau.ac.il> il <f139994 at ccsg.tau.ac.il> writes:
>I have a simple question: is it possible to store vegetative culture of
>B.subtilis in +4C (on the plate / liqued medium), how long?
>Thank you in advance.
>B.subtilis do not like 4C storage - they die. You can store them on plates
wrapped in parafilm or like for a number of weeks after they have sporulated.
We store spores on slopes at 4C for long periods(years). Simply make a slope,
inubate for a few days. Check under the microscope that youhave a good number
of spores, then seal it tight and store at 4C.
All the best
Mark Smith | The saying is sure and worthy of
Dept. of Biochemistry | full acceptance, that Christ Jesus
University of Sydney | came into the world to save sinners.
Sydney, NSW, 2006 | And I am the foremost of sinners.
m.smith at biochem.usyd.edu.au | 1 Timothy 1:15