Pausing of the prokaryotic replisome.

[Jeffrey Moseley]

My question concerns the pausing of the replisome in E. coli during 
co-ordinated leading and lagging strand synthesis.  Is due to a lag time in 
synthesis of a new RNA primer on the lagging strand, or is it because of the 
dissociation of the replisome from the lagging strand on encountering the 
5-prime end of the previous Okazaki fragment?

Thanks.

Jeff Moseley
UCLA.