Mark Fuller <mark_fuller at qmgate.anl.gov> writes:
>Does anyone have some general guidelines for inhibiting electron
>transport? I plan to work with rotenone antimycin A and
>2-N-heptyl-4-hydroxyquinolane-N-oxide and pure cultures of bacteria.
>What is the recommended concentrations for these compounds?
Of these three only HQNO (10-50 microM) worked in E.coli
in my hands. I tried up to 0.1 mM rotenone, but to no avail. These
were wild-type strains, though.
You might want to try piercidin (sp?) and/or amytal. Amytal
(5 mM) was succesfully used by M. Eisenbach in E.coli. You'll need a
special permision to use it (it's a barbiturate).
>Are there any special culture conditions that should be maintained
>to allow these compounds to work?
Stationary-phase E.coli have cyanide-resistant respiration and a
bunch of other nice features. You will be better of starting with log-
phase cells.
>Any advise, especially refererences, would be greatly appreciated.
Let me know, what exactly you're looking for. I'll have to dig
out of the snow and get to my desk...
>Mark Fuller, Ph. D.
Michael Galperin galperin at biotek.mcb.uconn.edu
Molecular and Cell Biology 75304.226 at compuserve.com
Univ. of Connecticut U-125 (860) 486-1898 (lab)
Storrs, CT 06269-3125 (860) 486-4331 (fax)
