purifying Thiobacillus/Cyanobacteria

[Paul . :-]

I am currently, trying my hardest, to purify enviromental
samples of halophilic Cyanobacteria, and samples of 
Thiobacillus ferrooxidans from a stream contaminated with 
acid mine drainage.

Problem being, nothing quite works the way others have reported.

So I was wondering if anybody here purfied any of these 
and could give me some guidance 'cause I need help

Paul.