Ori of pUCs and CloneWorks

Paul Morcos paulm at ucla.edu
Mon Apr 1 02:18:52 EST 1996

Hi Gilles,

The sequence of pUC vector origins are outlined in the GenBank files.  
Our lab is looking at a great new cloning and primer package called 
CloneWorks which allows you to load molecules like pUC18 etc.  One cool 
thing is that you can preview the files you load....like the GenBank 
file associated with it.  You can both see the origin outline in a 
graphic and see exactly what sequence it is from the GenBank file.  I 
hope this helps.  You can get a demo version of cloneworks from their 
site....http://www.cloneworks.com/anteater.  I think anteater is the 
name of the software company....must be UCI grads!
                        good luck
                      Dr. Paul Morcos

Gilles VACHON wrote:
> Hi all,
> I am looking for the sequence of origin of replication of pUC-like plasmids
> (especially pGEM vectors).
> Does someone on the net have this information or can tell me where to find it?
> Thanks
> Gilles Vachon
> Laboratoire de Genetique Moleculaire des Plantes,
> UMR 5755, CERMO
> Universite J. Fourier, BP 53X
> Tel: (33) 76 63 56 58
> fax: (33) 76 51 43 36
> e-mail: vachon at bio.grenet.fr

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