Growing MEthanosaeta concilii GP6

PATEREKJR paterekjr at aol.com
Thu Apr 4 10:28:53 EST 1996

If you added the iron and a reductant like Na2S(9H2O) while the medium is
still hot, then FeS can form and precipitate.

I add the filter sterilized Na2S(9H2O) solution to Hungate Roll tubes
prior to use after the medium has cooled to room temperature.

Agar roll tubes are held at approximately 50C in a water bath to keep the
agar molten, and the Na2S(9H2O) solution is injected just prior to
inoculation and rolling.

I have rarely had a black ppt. form in my medium.  Please contact me
directly if you have any other questions or problems, and I will be glad
to try to help.

J. Robert Paterek, Ph.D.
Manager, Environmental Biotechnology
Institute of Gas Technology
1700 S. Mt. Prospect Rd.
Des Plaines, IL   60018
paterek at igt.com

More information about the Microbio mailing list

Send comments to us at biosci-help [At] net.bio.net