My electroporation of bacterial cells didn't work.
I used the BIO RAD E coli gene pulser which lacks the ability to alter
Their self test indicates the machine is OK.
The cells are XL-Blue F`(300ul) at 2.5kV in a 0.2cm gap cuvette (Cold)
The plates are 100ug/ml carbenecillin.
No colonies. nothing with my ligation mixes. I thought too high in salt.
Then I tried Supercoiled puC18. No colonies when I added 5 ul of a
.01ng/ul solution of pUc18 which came from Stratagene.
I even bought some Stratagene electro comp cells and they didn't work either.
Why isn't my electroporation working?
Peter Emanuel Ph.D.
University of Maryland
School of Medicine