Dear Colleagues:
I am trying to confirm whether a protein I am working on
is located in the periplasmic fraction or not. I would
like to know what is the best method to separate cytosolic,
membrane and periplasmic fraction of E. coli. I found some
groups use low concentrations of Triton X-100 to disrupt
outer membrane and thus allow the periplasmic fraction released.
Is this a commonly acceptable method ? In addition, what can be
used as a periplasmic marker so that I can have a positive control.
Is cAMP phosphodiesterase a good marker for this purpose ?
Thank you very much for providing the information to me.
HY CHANG
Dept. Mol. Biol.
CGMC, TAIWAN
