mbras at s-crim1.dl.ac.uk (R.A. Skuce) wrote:
>>>> Your query was:
> corynebact
>>> _________________________________________________________________
>> Diagnostics Server www.atcc.org returns the following diagnostics:
> MEDIA: Search produced no result.
> _________________________________________________________________
>> The selected databases contain 6 documents matching your query:
> _________________________________________________________________
>> 1: Corynebacterium sp. ATCC 43771 R. Yanagawa and Y. Kudo 5-R14
> (Corynebact
>> 2: Rhodococcus sp. ATCC 21521 Takeda Chem. Ind., Ltd. IFO 12926
> (Corynebact
>> 3: Arthrobacter uratoxydans ATCC 21750 Noda Inst. Sci. Res. U-8
> (Corynebact
>> 4: Corynebacterium sp. ATCC 21684 Takeda Chem. Ind., Ltd. CF-03
> (Corynebact
>> 5: Rhodococcus rhodochrous ATCC 21766 Hasegawa Co., Ltd. MC-1-1
> (Corynebact
>> 6: Aureobacterium barkeri Collins et al. ATCC 15954 J.V. Bhat 7
> (Corynebact
>>> _________________________________________________________________
>> [IMAGE]
>> This page was generated by SFgate 4.0.18.
>I would like to hear from anyone who could tell me the best
>media to grow Corynebacteria in/on, specially C. tuberculostericum.
>I would also like to know the natural habitat of these bacteria.
>>many thanks
>>David Brittain
David,
Corynebacterium (and other coryneform bacteria) are widespread in the
soil environment, but many are also pathogenic to man (ie., C. diptheria
cause diptheria, and Rhodococcus equi can cause lung infections). I'd
suggest starting simple -- try 0.1 strength to full strength tryptic soy
broth/agar. This is not SELECTIVE media, but it should support the
growth of these organisms quite well. For selecting these organisms,
try:
-----------------------------------------
Medium 2 (from Handbook of Media for Environmental Microbiology, R.
Atlas, 1995, CRC Press, p. 273)
composition per L:
agar 15 g
glucose 10 g
LiCl 5 g
pancreatic digest of casein 4 g
yeast extract 2 g
TRIS-HCl buffer 1.2 g
NH4Cl 1.0 g
MgSO4€7H2O 0.3 g
Polymyxin sulfate sol'n 10 mL
NaN3 sol'n 10 mL
pH 6.9 ± 0.2
Polymyxin sulfate sol'n
Polymyxin sulfate 0.04 g/10 mL
filter sterilize. use immediately
NaN3 sol'n
NaN3 (sodium azide) 2.0 mg/10 mL
filter sterilize. use immediately
CAUTION: NaN3 is toxic. Azides react
with metals and disposal must be highly
deluted
Media Prep
Add all ingredients except solutions to 980 mL
water, mix and heat to dissolve. Autoclave.
Cool to 45-50°C. Add solutions aseptically and mix.
Pour into petri plates.
For the selective isolation and cultivation of Corynebacterium species
------------------------------
Hope this is of use. If you can find a copy of this book, it is a great
reference tool.
-Mark Fuller, Ph.D.
