Hello,
Now then, what species of Pseudomonas are you interested in and is it
cytochromes c in general you want to look at or one in particular? Both
Pseudomonas aeruginosa and Pseudomonas stutzeri produce a wide range of
cytochromes under various growth conditions.
If you're interested in the broad range of cytochromes, a simple way is to
prepare a total soluble extract, or a periplasmic extract by spheroplasting,
load it onto a large ion exchange column (e.g. DEAE cellulose) and run a NaCl
gradient of say 0-400mM. Cytochromes c will elute at various places as
coloured bands and this is usually enough purification for a crude spectrum.
You can clean up fractions of interest by a further column, such as gel
filtration.
I can supply further details, if you can be more specific.
Neil Saunders
