We have 2 immediate openings for persons with strong
backgrounds bacterial genetics or molecular biology/
biochemistry
* The first project is in bacterial pathogenesis,
specifically the investigation of complement-mediated
bacterial killing. It involves expression of
recombinant C9 molecules in E. coli for export into the
periplasm and identification of cellular constituents
required for C9-mediated killing using genetic
techniques. This position requires a person with strong
background in bacterial genetics (specifically Gram-
negatives).
* The second project involves mutagenesis of complement
protein C9 to introduce cysteine residues for site-
specific labeling. Cys mutants will be labeled with
Nano-gold for electron microscopy and image
reconstruction studies, with spin labels for ESR
spectroscopy, and bifunctional crosslinking reagents for
analyses of nearest C9 neighbors in the C5b-9 complex
(MAC). This position requires a person with strong
background in recombinant DNA techniques including some
skill in protein purification and cell culturing
(preferably with insect cells). Knowledge of ESR
spectroscopy or electron microscopy is not required for
this position, although the individual will have the
opportunity to learn such techniques.
Please send inquiries about these positions to:
Alfred F. Esser, Ph.D., or to
Joan Stader, Ph.D.
Division of Cell Biology & Biophysics
School of Biological Sciences
University of Missouri
Kansas City, MO 64110
Tel: (816) 235-5316
FAX: (816) 235-5158
E-mail: AESSER at CCTR.UMKC.EDU
