Getting no ligation whatsoever with different ligases from different
companies (BRL, Promega, Boehringer, Pharmacia), gel purified (Sigma low
melt), single cut vector religating on itself, blunt (A-overhangs into
T-vectors) and sticky end ligations,addition of ATP into ligation
reactions, ligating at 12 degree and 15 as well. What to do, any
suggestions? Please help. Oh yes, cells are competent.
Henry Wong
MID, Universtiy of Calgary
email: hwong at acs.ucalgary.ca
