Bactericidal activity of EDTA

Reno Pontarollo Reno.Pontarollo at dres.dnd.ca
Fri Jun 14 13:40:11 EST 1996

Rene, I used 0.1 M EDTA to kill E. coli JM105.  This kept the cells intact to use in a lysozyme 
assay and worked well.

Basic protocol was to resuspend the culture in an equal volume of EDTA, gently agitate for 5 
min, pellet, and resuspend in Tris pH 7.4.
Good luck.
Reno Pontarollo Ph.D.
Medical Countermeasures Section, Defence Research Establishment Suffield (DRES)
Medicine Hat, Alberta, Canada
EMAIL:  Reno.Pontarollo at dres.dnd.ca  BUS:403-544-7674  FAX:403-544-3388

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