We filter large numbers of tissue homogenates through 0.45 micron
syringe filters for viral assays. Recently we had a bacterial contaminant
in our tissue culture cell lines (fish cells) that was identified by
Biolog rapid identification kits as Sphingobacterium multivoram. We have
been able to pass broth cultures of this bacterium through 0.45 micron
filters from three different manufacturers.
Does anyone know anything about this particuler species? Does it
readily produce L forms? Is it unusually small? Does it produce spores
(Bergeys says not)?
Has anyone had experience with bacteria that can be repeatedly
passed through a 0.45 micron filter?
Steve Lord slord at uoguelph.ca
