Anaerobic Medium?

Michael Casey Michael.Casey at mbox.fam.admin.ch
Tue Jun 25 18:34:33 EST 1996

I am studying the rate of growth of Clostridium tyrobutyricum in acid medium
and have found them to be very sensitive to the presence of oxygen. I follow
the growth rate by measuring the turbidity at 650 nm. I have tried using
different techniques to reduce the oxygen content of the medium but with 
little success. 

Gassing with CO2 does not reduce the oxygen content sufficiently.

The addition of cysteine to the medium is also not sufficient but the
presence of sodium thiogllycolate inhibits growth. 

The addition of Oxyrase, a commercial enzyme preparation which removes 
oxygen, causes turbidity and prevents accurate measurement of growth.

Does anyone have an idea how I could reduce the oxygen content of
the medium without causing turbidity?

Thank you.

Michael Casey
Swiss Federal Dairy Research Institute
3097 Liebefeld
Tel: (+41) 31 323 81 79
FAX: (+41) 31 323 82 27
email Michael.Casey at fam.admin.ch

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