Hello: Why not use BBl Gas Pak PLus(catalogue # 4371040), a product
produced by Becton Dickison Microbiology Systems, Cockeysville, Maryland,
USA (tech information: (800-638-8663). Each box contains 10 aanaerobic
atmosphere generating envelopes., Hydrogen, generated from a sodium
borohydride tablet following the addition of water to the envelope,
combines with the oxygen in the presence of a palladium catalyst to
produce water (the envelope & the cultures are enclosed in a tightly
sealed/clamped jar). 4 -10 % Co2 is also generated to stimulate the growth
of anaerobes( the Co2 is produced by the combination of citric acid &
sodium bicarbonate). The system is easy to use & not expensive. Tach
service phone: (800)638-8663. ASk tech services for details about o2
concentration. It is certainly low enough to support most anaerobes. You
should also purchase methylene blue anaerobic indicator strips to monitor
the performance of the envelope/jar system. Hope this answers your need.
Good luck
Ken Inglima
